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10.1016/j.freeradbiomed.2007.11.008 Free Radic Biol Med

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doi: 10.3390/fermentation3010001

Essential fatty acids are essential for human health and life, but the body cant synthesize it itself and must rely on the food supply, they are unsaturated fatty acids

After incubation in the blocking buffer (3% BSA in PBS) for 1 h at room temperature (blocking), oocytes were incubated with Rhodamine phalloidin (Invitrogen, Cat# R415) diluted 1:50 in the blocking buffer for 40 min at room temperature

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